RESUMO
Historically, major extirpative surgery for control of head and neck cancer developed well in advance of reconstructive procedures that could provide acceptable functional and cosmetic rehabilitation. Introduction of cutaneous axial and random flaps--followed more recently by the description of musculocutaneous flaps--represented major advances in reconstructive techniques.
Assuntos
Neoplasias de Cabeça e Pescoço/cirurgia , Retalhos Cirúrgicos , Adulto , Idoso , Cadáver , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Músculos Peitorais/irrigação sanguínea , Músculos Peitorais/cirurgia , Pele/irrigação sanguínea , Artérias Torácicas/anatomia & histologiaRESUMO
Short term microculture systems which measure murine lymphocyte proliferative responses to mitogens are well established. We demonstrate here that these microculture methods are not suitable for antigen-induced responses because of the high levels of murine lymphocyte proliferation in control cultures associated with the use of fetal calf serum or human serum. We also show that this problem can be eliminated with the use of a combination of 5% horse serum and 5 X 10(-5) M mercaptoethanol. We describe an antigen-induced murine lymphocyte proliferation microculture system in which good stimulation indices are achieved and the lymphocyte proliferation in control cultures remain at a low level throughout the 7 day culture period.
Assuntos
Antígenos , Sangue , Ativação Linfocitária , Mercaptoetanol/farmacologia , Animais , Bovinos , Células Cultivadas , Relação Dose-Resposta Imunológica , Feminino , Feto , Cavalos , Humanos , Camundongos , Camundongos Endogâmicos BALB C , Gravidez , Estreptodornase e Estreptoquinase/imunologia , TuberculinaRESUMO
The inheritance of responsiveness to histamine-sensitizing factor of pertussis bacilli was examined in (SWR/J x CBA/J)F(1) x CBA/J backcross mice. We found that about half of the backcross mice inherited this responsiveness in both sexes. There was no linkage between the inheritance of responsiveness to histamine-sensitizing factor and the H-2(q) locus from the sensitive SW/J progenitor.
